Cells being placed in an Alvetex well insert

Get your 10% discount on all Alvetex Products

Orders placed during July 2021

To celebrate over 10 years of Alvetex research and save you money, we are offering a 10% discount on all Alvetex products this July giving you the chance to try our award winning 3D cell culture scaffold for less.

Simply place your order and a discount will be automatically applied.

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Alvetex well plate formats

Alvetex-formats-1

  • Ideal for 3D culture in the top half of the scaffold, or layered above
  • Cells are fed from the top of the scaffold only
  • Useful for when restricted cell penetration is required
  • An option for use with expensive cells, reducing cell number (e.g. cell transfection)
  • Allows high throughput applications in 3D

Alvetex well insert formats

Alvetex-formats-2

  • Enables cells to be fed from above and below simultaneously
  • Ideal for longer term 3D culture
  • Growth of cells at the air/liquid interface
  • Readily transfer 3D cultures to a fresh plate
  • Enables co-culture studies (2D+3D, or 3D+3D)

Alvetex well insert holder

Alvetex-formats-3

  • Reduces frequency of media changing as up to 95 mL of medium
  • Can be used to support a single 3D culture
  • Ideal for maintaining long term 3D culture experiments of up to several weeks
  • Facilitates the use of a magnetic stirrer to increase media circulation if required

Alvetex perfusion plates

Alvetex-formats-4

  • Inter-changeable Luerlocks can be adapted to a range of tubing diameters
  • Tissue fragments, 3D cultures in Alvetex well inserts and 2D cells can be maintained in the perfusion plate (for 2D cells, the plasticware may require coating with the appropriate cell culture reagent to promote cell adhesion)
  • Unidirectional flow between separate wells allows the study of the interaction between cell populations grown in separate wells through the release of paracrine factors

What is Alvetex?

Alvetex is a porous polystyrene membrane that can be used to culture cells in three dimensions. Its inert, consistent structure is 90% porous, providing maximal space for cell growth. 

Cells grow and divide occupying the 3D space within Alvetex Scaffold (or on top of Alvetex Strata), maintaining their natural shape and forming complex interactions with one another in a manner that closely mimics normal growth in tissues. The cells may lay down extra-cellular matrix which often leads to the formation of “mini-slab” tissue-like structures.

Typical mammalian cells are around 10-25 μm in size and are rarely further than 0-50 μm from another cell or 100-200 μm from a source of nutrients via a blood capillary. By recreating this complex cellular organisation and environment exprienced by cells within their native tissues, Alvetex 3D cell culture enables more accurate investigation into the study of cell behaviour and function compared with conventional 2D model systems.

Alvetex is compatible with a broad range of standard molecular, cellular and histological techniques, and it is available in a variety of formats, including multi-well plates and inserts.

Discover more:

Download our Alvetex brochure (PDF)

Alvetex-examples

 Image: Examples of cells grown in Alvetex, visualised by various techniques. (A.) Scanning Electron Micrograph of cells grown throughout Alvetex Scaffold. (B.) Murine keratinocytes grown in Alvetex Scaffold; fixed, embedded, Paraffin sectioned, stained and viewed by bright field microscopy. (C.) Hepatocarcinoma HepG2 cells grown on Alvetex Scaffold. Triple fluorescent stained and viewed by confocal microscopy. (D.) Caco-2 cells in 3D growth on top of Alvetex Strata.

Get your 10% discount on all Alvetex orders placed in July 2021.

Go to Alvetex products →

 

Note: This 10% discount is off the RRP of Alvetex products and is only available for direct-sales customers only.